Screen Readers Skip to Content

Altered Milk Protein Can Deliver AIDS Drugs to Children

Author: Penn State
Published: 11th Nov 2014 - Updated: 18th Dec 2014
Peer-Reviewed Publication: N/A
Additional References: HIV and Aids Publications

Summary: Method of altering a protein in milk to bind with antiretroviral drug improves treatment for infants with HIV/AIDS.

Main Document

That's critical because an estimated 3.4 million children are living with HIV/AIDS, the World Health Organization reports, and nine out of 10 of them live in resource-limited countries in sub-Saharan Africa, where effective antiretroviral treatments still are not widely accessible or available. International medical experts believe less than a third of affected children worldwide receive an antiretroviral drug.

Complicating treatment is that most antiretroviral drugs are not well tolerated by very young children.

One of the most commonly prescribed antiretroviral drugs for treating and preventing HIV infection, Ritonavir, has undesirable side effects and important oral-delivery problems. Its physicochemical properties challenge its administration to infants, explained Federico Harte, associate professor of food science.

"Ritonavir has a high hydrophobicity and low solubility in water, which lead to a low dissolution rate in the gastrointestinal fluid and, hence, to insufficient bioavailability. The liquid formulation used to treat infants over one month of age contains 43 percent ethanol and has an awful flavor that has been described as bitter-metallic, medicinal, astringent, sour and burning," he said.

"Moreover, when coming into contact with the stomach mucosa, Ritonavir causes nausea, vomiting and diarrhea. Therefore, we need to develop alternative pediatric formulations of Ritonavir and overcome its poor water solubility to improve its oral administration to infants and children."

To solve that problem, Harte looked to a group of proteins in cow's milk celled caseins.

Casein proteins form spherical aggregates called casein micelles, which are responsible, incidentally, for the white color of milk. The casein micelles in mammals' milk are natural delivery systems for amino acids and calcium from mother to young, and Harte reasoned, might deliver Ritonavir molecules as well.

"I have been working with bovine casein micelles for a few years now, and we have investigated the structure and functionality of these proteins," he said. "What we found is these micelles are able to carry molecules that have very little solubility in water, that have low molecular weight and that are very hydrophobic - such as Ritonavir."

Significantly, Harte discovered in his research - recently published online in the Journal of Pharmaceutical Research - that subjecting milk to ultrahigh-pressure homogenization enhances the binding properties of the casein micelles. In previous studies, he learned that casein micelles could be bound to triclosan - an antimicrobial used in deodorants -- and vitamin D, which is added regularly to skim milk.

Normal milk is homogenized at 10 to 15 megapascals, he pointed out.

Milk in this research was homogenized at between 400 and 500 megapascals, disassociating the casein micelles and improving the protein's binding qualities to attach to drug molecules.

"As a result of this enhanced binding of molecules, we believe a milk powder containing Ritonavir can be used as baby formula, providing a transport system for a drug that is not very soluble in water. Right now we are running tests, and we are in the final stages of an experiment in which we gave three different formulations to piglets," Harte said.

"We are taking blood serum samples every three hours to study the kinetics of the drug in the piglets," he said. "The hope is that -- and we don't have the data yet -- we find that the Ritonavir is being adequately delivered by the protein in milk. So if that works, I think we are pretty close to having a formulation that can be used with hydrophobic drugs."

Harte noted that with his proposal for research funding he included a letter from a pediatrician at St. Jude Children's Research Hospital describing the challenge of orally administering Ritonavir to infants and young children.

"She has been treating patients with AIDS, and the awful flavor and ethanol content of Ritonavir were big issues for her," he said. "I am hopeful that this may lead to an application that works against AIDS. We have not done any clinical trials yet, so we will need to get data from those trials to say for sure."

Post to Twitter Add to Facebook

Latest HIV and Aids Publications

The above information is from our reference library of resources relating to HIV and Aids that includes:

Rainbow-colored LGBT letters. thumbnail image
Online toolkit helps parents improve communication with gay and bisexual sons about behaviors to help them stay healthy.
Publish Date: 13th Nov 2022 - Updated: 5th Jan 2023
FDA Approves Trogarzo (ibalizumab-uiyk) for Patients with Limited HIV Treatment Options thumbnail image.
U.S. FDA approves Trogarzo for adult patients with HIV who have tried multiple medications and whose HIV infections cannot be treated with other therapies - multidrug resistant HIV, or MDR HIV.
Publish Date: 7th Mar 2018
Undetectable/Untransmittable: Gay - Bisexual Men Skeptical But Attitudes On The Rise thumbnail image.
Hunter CHEST study sought to examine how accurate gay and bisexual men perceive this message to be by surveying more than 12,000 men across the United States.
Publish Date: 16th Jan 2018
Transgender Women Take Triple the Number of HIV Tests As Trans Men thumbnail image.
Study revealed HIV testing among transgender adults was higher in those who identified as female, were African-American or Hispanic, or had a history of incarceration.
Publish Date: 1st Nov 2017

1How Many Genders Are There?
Alphabetical list of gender identities.

2Transgender Reporting Guide
How to write about transgender people.

3Glossary of Sexuality Terms
Definitions of sexual terms & acronyms.

4Glossary of Sexual Identities
Definitions of gender related terms.

5Am I Gay? Questions to Ask
Think you may be gay or bisexual?

• Submissions: Send us your coming events and LGBTQ related news stories.

• Report Errors: Please report outdated or inaccurate information to us.

• (APA): Penn State. (2014, November 11). Altered Milk Protein Can Deliver AIDS Drugs to Children. Retrieved May 24, 2024 from

• Permalink: <a href="">Altered Milk Protein Can Deliver AIDS Drugs to Children</a>